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  • Ovulation Test Instructio​ns
One Step LH Urine Ovulation Test (Strip) Instructions


Ovulation is the release of an egg from the ovary. The egg then passes into the fallopian tube where it is ready to be fertilized. A baby is conceived when the male sperm successfully fertilizes the female egg. When a woman is about to ovulate, her body releases a large amount of a hormone called L.H. (Luteinising Hormone). L.H. is always present in your urine but the levels increase (surge) in the middle of your cycle, causing you to release an egg from the ovary.


The One Step Ovulation Test is an immunochromatographic    in-vitro assay for the qualitative and semi-quantitative determination of the human luteinizing hormone (LH) in urine to predict the time of ovulation in women. The test detects the sharp increase in LH concentration in urine, the so called “LH surge” which precedes ovulation. Conception is most likely to occur within 36 hours following the LH surge.


Each pouch contains one immunochromatographic test. Each test contains a membrane with anti-LH antibodies and conjugates, in a stabilizing matrix containing proteins and sodium azide.


Store below 30C; do not freeze.


•    The One Step Ovulation Test is for in vitro diagnostic use only.
•    Elevated concentrations of Human Chorionic Gonadtropin (HCG) interfere with LH testing. Do not test samples from pregnant women and people with pathologic conditions causing higher HCG levels.
•    The One Step Ovulation Test is not designed to prevent conception. As sperm can survive for 72 hours you might still become pregnant if you had intercourse before you detected your L.H. surge.


First, you must determine the length of your menstrual cycle.  This is the number of days from the first day of your menstrual bleeding to the day before your next bleeding begins again, count the first day of bleeding as day 1. Calculate what the usual length of your menstrual cycle has been over the last few months. Once you have worked out the length of your cycle refer to the chart below to determine on which day of your menstrual cycle you should begin testing.

Cycle Length

Day to begin Testing

21-22 days

Cycle Day 6

23-24 days

Cycle Day 7

25 days

Cycle Day 8

26 days

Cycle Day 9

27 days

Cycle Day 10

28 days

Cycle Day 11

29 days

Cycle Day 12

30 days

Cycle Day 13

31 days

Cycle Day 14

32 days

Cycle Day 15

33 days

Cycle Day 16

34 days

Cycle Day 17

35 days

Cycle Day18

36 days

Cycle Day 19

37 days

Cycle Day 20

38 days

Cycle Day 21


If your cycle is normally 28 days, the cycle chart above indicates you should begin testing on Day 11.


If your cycle is shorter than 21 days or longer than 40 days, consult your doctor. If you do not know your cycle length, you may begin the test 11 days after your first period since the average cycle length is 28 days. Perform 1 test each day until the LH surge has been detected.


Once you have identified what day you should begin testing you should then begin to collect your urine on a daily basis.

1.    Do not use first morning urine samples as LH is synthesized in your body early in the morning. It will not show up in your urine until later in the day.
2.    The best time to collect your urine is between 10am - 8pm. Pick a regular time that suits you best.
3.    Collect urine at about the same time each day.

Reduce liquid intake about 2 hours before collecting your urine as a diluted urine sample can prevent the test from detecting LH surge.


1.    Read the instructions thoroughly before you begin.
2.    Do not open the foil pouch until you are ready to begin the test.
3.    Make sure you have a watch, clock or timer ready.
4.    Allow urine samples and test kit to reach room temperature before testing (approx 20 mins).


1.    Determine the day to begin testing.
2.    Collect urine sample in a clean and dry container.
3.    To begin testing, open the sealed pouch and remove the strip. Do not remove the strip until you are ready to begin testing.
4.    With the arrows pointing downwards towards the urine, place the test strip vertically (straight) into the urine sample, for at least 10 seconds. DO NOT allow the urine to go above the MAX (maximum) level line.
5.    Remove the strip from the urine and place on a clean, dry surface. For best results you should read the results at 10 minutes.
6.    Wait for coloured bands to appear. Depending on the concentration of LH in the urine specimen, positive results may be observed in as short as 40 seconds. However, to confirm negative results, the complete reaction time of 10 minutes is required.


To determine your result you must compare the colour intensity of the test band to the control band.

Positive for L.H. surge
If two colour bands are visible and the test band is of almost equal or greater colour intensity (darker) than the control band, this is a positive result and a good indication that the L.H. surge is occurring. You should ovulate within the next 24-36 hours. Sexual intercourse is advised at anytime after the first positive test.

Negative for L.H. surge
If two bands are visible but the test band is of a less intense colour (paler) than the control band or cannot be seen, this means the L.H. level is at or near its normal level and that the surge is not in progress. You should continue with daily testing.

Invalid result    
If no control band appears within 5 minutes, the result is invalid and should be ignored. A visible control line is needed in all cases to confirm a proper test result. Repeat test with a new test kit.

Manufactured by: AI DE DiagnosticaCOo. Ltd., 141 Zhu Zhou Road, Quingdao High-Tech Industrial Park, 266101 Shandong, P.R. China
Distributed in the UK by Home Health UK Ltd.